Isolation of total RNA was performed using Trizol reagent for liver biopsies (Invitrogen, Paisley, Scotland, UK) and using RNABee reagent for HepG2 cells (AMS Biotechnology, Abingdon, UK). Reverse ...

Total RNA was extracted from ∼30 adult flies using a commercial TRIZOL reagent (15596026, Invitrogen) and purified with DNA- free kit (AM1906, Ambion) according to the manufacturer’s protocol.

We include a new reagent with favorable properties, 5- nitroisatoic anhydride (5NIA). This study clarifies the importance of carefully designed software in reading out SHAPE experiments using ...