After a brief rinse in Tris-buffered saline with Tween™ 20 detergent (TBST), goat anti-rat IgG (H + L) HRP-conjugated secondary antibodies (Invitrogen, Waltham, MA, USA) were used in 1:5000 dilution ...

Membranes were blocked in 5% skim milk in TBST for 1 hour at room temperature, and then incubated with primary antibody in 3% BSA or 5% skim milk in TBST at 4°C overnight. Membranes were washed 3 x 10 ...

Following three washes with TBST, peroxidase-conjugated secondary antibodies [all at 1:10,000; for anti-Ptbp2 and anti-Histone H3: mouse monoclonal anti-rabbit (211-032-171, Jackson ImmunoResearch); ...

1 The Second Clinical Medical College of Henan University of Chinese Medicine, Zhengzhou, China 2 Henan Province Hospital of Traditional Chinese Medicine, The Second Affiliated Hospital of Henan ...

Afterwards, the membranes were blocked in TBST (1 mM Tris–HCl, pH 7.4, 150 mM NaCl, 0.05% Tween-20) containing 5% BSA for 1 h and subsequently incubated overnight at 4°C with diluted primary ...

To address the unmet need for a cure for RTT, the therapeutic potential of adult Neural Precursor Cells (NPCs) was ...

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