Following the TBST washing, the membranes were separately incubated with primary and secondary antibodies at room temperature for a duration of 2 h. Subsequently, TBST was used for further cleaning ...

Drosophila melanogaster is a widely used model organism in cancer research due to its genetic tractability and the high degree of conservation of key cancer-related pathways (Bilder et al, 2021; Liu ...

The membranes were blocked with a TBST solution containing 5% skim milk powder at room temperature for 1.5 h, followed by overnight incubation with the appropriate primary antibodies at 4°C. After ...

Asthenoteratozoospermia, a prevalent cause of male infertility, lacks a well-defined etiology. DNAH12 is a special dynein featured by the absence of a microtubule-binding domain, however, its ...

Pulmonary fibrosis is excessive scarring of the lung tissues. Transforming growth factor-beta (TGF-β) has been implicated in pulmonary fibrosis due to its ability to induce the ...

This fundamental work provides evidence that glutamate and GABA are released from different synaptic vesicles at supramammillary axon terminals onto granule cells of the dentate gyrus. The study uses ...

3.2 Effects of TBST on cycle of SMMC-7721 cells After treated with TBST for 24 h, the cycle of SMMC-7721 cells had greatly changed. With the increase of TBST concentration from 0 to 400 μg/mL, the ...

Membranes were then blocked at RT with gentle shaking for 1 hour in 5% milk in TBST [1× TBS (#1706435, Bio-Rad) + 0.1% Tween-20 (#P7949, Sigma-Aldrich)]. After blocking, membranes were incubated in ...

The membranes were blocked with TBST containing 5% bovine serum albumin (BSA) for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C. After being washed three times with ...

This study presents an innovative approach for urban functional zoning and planning based on artificial intelligence (AI) techniques. Utilizing multi-modal remote sensing data and geographic ...

After washing in TBST 3 times for 5 minutes each, samples were probed with 5 µmol/L TAD1 in 1× BSA/TBST for 1 hour. After washing in TBST 3 times for 10 minutes each, the fluorescence intensity of ...